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Separation of 1,3-propanediol from glycerol-based fermentations of Klebsiella pneumoniae by alcohol precipitation

GAO Sujun, SUN Yaqin, XIU Zhilong

《化学科学与工程前沿（英文）》 2007年第1卷第2期页码 202-207 doi: 10.1007/s11705-007-0037-1

摘要： The separation of 1,3-propanediol from the glycerol-based fermentation broth of plays an important role during the microbial production of 1,3-propanediol. In this paper, the separation of 1,3-propanediol from fermentative broth by a combination of ultrafiltration and alcohol dilution crystallization was investigated. The broth was first filtered by ultrafiltration, and 99% of cells, 89.4% of proteins and 69% of nucleic acids were removed. The obtained broth was further condensed by vac uum distillation, and then alcohol was added. The macromolecular impurities, such as nucleic acids, polysaccharides and proteins, were precipitated, and inorganic and organic salts were crystallized. The optimal volume ratio of alcohol added to the condensed fermentation broth was determined to be 2:1. As a result, proteins, nucleic acids and electric conductivity decreased by 97.4%, 89.7% and 95.8%, respectively, compared with the fermentative broth. The influences of pH and water content in condensed broth on alcohol precipitation and dilution crystallization were also investigated. The experimen tal results indicated that alcohol precipitation and dilution crystallization was feasible and effective for the separation of 1,3-propanediol from actual fermentation broth.

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Early diagnosis and therapeutic choice of

Jing LI, Ying FU, Ji-Yao WANG, Chuan-Tao TU, Xi-Zhong SHEN, Lei LI, Wei JIANG

《医学前沿（英文）》 2010年第4卷第3期页码 308-316 doi: 10.1007/s11684-010-0103-9

摘要： Nowadays, pyogenic liver abscess (PLA) is still a common and severe intra-abdominal infection, and had emerged as the most common pathogenic bacteria worldwide in the past ten years. Our study aims to achieve an early pathogenic diagnosis and rational therapy modality for liver abscess (KLA) through clinical data analysis. A total of 197 inpatients in Zhongshan Hospital, Shanghai, diagnosed as having liver abscess between March 2001 and September 2009 were enrolled. Patients with monomicrobial infection were divided into two groups: patients with liver abscess (KLA group, =106) and those with non- liver abscess (NKLA group, =56). A retrospective analysis was made between these two groups on the aspects of underlying diseases, clinical characteristics, laboratory data, culture results, and imaging findings. To evaluate the effects of different medical interventions, monomicrobial KLA patients were further divided into four subgroups (percutaneous liver aspiration, aspiration plus antibiotics flushing, aspiration plus retained catheter, and aspiration plus antibiotics flushing and retained catheter), and corresponding therapeutic effects were analyzed. KLA was more likely to occur in patients with coexisting diseases such as diabetes mellitus (53.77% 25.00%, =0.001) and hepatic adipose infiltration (16.04% 5.36%, =0.029). Compared to NKLA group, clinical characteristics including abdominal pain (40.57% 57.14%, =0.044), hypodynamia (19.81% 46.43%, =0.001), and hepatomegaly (4.72% 14.29%, =0.033) were much milder, but with a higher fasting blood glucose level (7.84?±?0.36 5.76?±?0.30, =0.001) on admission in KLA group. In addition, KLA abscess often appeared singly in the right lobe of the liver with gas forming nature (32.88% 13.51%, =0.039), unsmooth rim (71.23% 40.54%, =0.002), and dynamic septum enhancement (41.10% 16.22%, =0.009). Compared to mono aspiration subgroup, additional antibiotic flushing could not further improve clinical outcomes of KLA patients ( >0.05); however, the retained catheter showed obvious advantage in reducing abscess diameter (34.38?±?3.25 mm 22.67?±?2.37 mm, =0.017). It can be concluded that the strong association with diabetes, milder clinical symptoms, and gas-forming nature in CT images makes early pathogenic diagnosis of KLA possible. Comparatively, ultrasonography-guided percutaneous liver aspiration with retained catheter may be the most rational intervention modality of KLA.

关键词： liver abscess Klebsiella pneumoniae computed tomography therapy

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2018—2019年我国北方地区奶牛临床型乳房炎源肺炎克雷伯菌的分子特征 Article

宋士凯, 何文娟, 杨大伟, Manar Benmouffok, 王瑶, 李基云, 孙城涛, 宋祥彬, 马士珍, 蔡畅, 丁双阳, 吴聪明, 沈张奇, 汪洋

《工程（英文）》 2022年第10卷第3期页码 146-154 doi: 10.1016/j.eng.2021.01.015

摘要：

肺炎克雷伯菌是奶牛乳房炎的重要诱因，常引起奶牛的产奶量降低和死亡率增高。目前尚缺乏奶牛乳房炎源肺炎克雷伯菌分子特征的研究数据，限制了对其在食品生产链中传播风险的评估。本研究于2018—2019 年从我国北方地区患临床型乳房炎奶牛中采集了6301 份奶样，共分离到183 株肺炎克雷伯菌，两年的平均分离率分别为3.03%和2.80%。与人类临床肺炎克雷伯菌分离株相似，奶牛临床型乳房炎源肺炎克雷伯菌分离株可分为肺炎克雷伯菌(KpI，143 株）、准肺炎克雷伯菌（KpII-B，37 株）和变栖肺炎克雷伯菌（KpIII，3 株）三个种群。在KpI 分离株中检测到超广谱β-内酰胺酶（ESBL）的编码基因bla_SHV-2a、bla_CTX-M-14和bla_CTX-M-15，以及可能与奶牛致病性和宿主适应性有关的编码基因clpC、lpfA、lacI、lacZ、lacY和fecABDEIR。KpI 菌株比KpII-B 和KpIII 菌株表现出更高的流行率以及抗生素耐药基因和毒力基因携带率，表明它可能对奶牛的危害更大。此外，还发现奶牛KpI菌株与人医临床分离的KpI菌株在种群结构上存在明显差异，且奶牛源KpI菌株中很少见到与人类侵袭性感染相关的基因，表明奶牛源KpI菌株对人类健康的威胁较小；奶牛KpII-B 分离株与人类感染分离株具有较高的核苷酸序列一致性，且它们携带固氮基因nif的比例都很高，表明奶牛和人类KpII-B 分离株与植物源性KpII-B 菌株之间存在一定关联。

关键词：临床型乳房炎肺炎克雷伯菌分子特征种群结构抗生素耐药性

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Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein

《医学前沿（英文）》 2022年第16卷第3期页码 378-388 doi: 10.1007/s11684-021-0840-y

摘要： Macrolide and corticosteroid resistance has been reported in patients with Mycoplasma pneumoniae (MP) pneumonia (MPP). MP clearance is difficult to achieve through antibiotic treatment in sensitive patients with severe MPP (SMPP). SMPP in children might progress to airway remodeling and even bronchiolitis/bronchitis obliterans. Therefore, identifying serum biomarkers that indicate MPP progression and exploring new targeted drugs for SMPP treatment require urgency. In this study, serum samples were collected from patients with general MPP (GMPP) and SMPP to conduct proteomics profiling. The Fc fragment of the IgG-binding protein (FCGBP) was identified as the most promising indicator of SMPP. Biological enrichment analysis indicated uncontrolled inflammation in SMPP. ELISA results proved that the FCGBP level in patients with SMPP was substantially higher than that in patients with GMPP. Furthermore, the FCGBP levels showed a decreasing trend in patients with GMPP but the opposite trend in patients with SMPP during disease progression. Connectivity map analyses identified 25 possible targeted drugs for SMPP treatment. Among them, a mechanistic target of rapamycin kinase (mTOR) inhibitor, which is a macrolide compound and a cell proliferation inhibitor, was the most promising candidate for targeting SMPP. To our knowledge, this study was the first proteomics-based characterization of patients with SMPP and GMPP.

关键词： severe Mycoplasma pneumoniae pneumonia children proteomics Fc fragment of the IgG-binding protein mechanistic target of rapamycin kinase inhibitor